Appeal point
It can quantify the shell density of small ocean plankton.
It is possible to understand changes in the body size and skeletal density of marine life in response to changes in the marine environment.
It is possible to understand changes in the body size and skeletal density of marine life in response to changes in the marine environment.
Summary
The present invention realizes the quantification of the shell density of minute ocean plankton having hard shells, which could not be achieved conventionally by using the microfocus X-ray CT, and has the following configuration.
1) Prepare a plurality of types of calibration reference samples that are made of calcium carbonate and have different densities. The calcite CT value for each is acquired in advance, and the calibration curve data showing the correlation with the density of each of the plurality of calibration reference samples is acquired.
2) Simultaneously irradiate at least one of the calibration reference sample and the measurement target with X-rays from the same X-ray source, and obtain primary CT image data of each of the calibration reference sample and the measurement target.
3) The fluctuation of the X-ray absorption amount in the primary CT image data of the measurement object is corrected based on the primary CT image data of the calibration reference sample to obtain the secondary CT image data.
4) The calcite CT value obtained based on the secondary CT image data of the measurement object is combined with the calibration curve data to measure the density of the measurement object.
By using this method, it becomes possible to accurately detect changes in the body size and skeletal density of individual organisms due to changes in the marine environment, particularly global warming and ocean acidification. Therefore, it is possible to further deepen the understanding of the mutual relationship between environmental changes and living things.
1) Prepare a plurality of types of calibration reference samples that are made of calcium carbonate and have different densities. The calcite CT value for each is acquired in advance, and the calibration curve data showing the correlation with the density of each of the plurality of calibration reference samples is acquired.
2) Simultaneously irradiate at least one of the calibration reference sample and the measurement target with X-rays from the same X-ray source, and obtain primary CT image data of each of the calibration reference sample and the measurement target.
3) The fluctuation of the X-ray absorption amount in the primary CT image data of the measurement object is corrected based on the primary CT image data of the calibration reference sample to obtain the secondary CT image data.
4) The calcite CT value obtained based on the secondary CT image data of the measurement object is combined with the calibration curve data to measure the density of the measurement object.
By using this method, it becomes possible to accurately detect changes in the body size and skeletal density of individual organisms due to changes in the marine environment, particularly global warming and ocean acidification. Therefore, it is possible to further deepen the understanding of the mutual relationship between environmental changes and living things.
Schematic image
The figure is an example of the calibration curve data, and the plot alpha is the actual measurement value for the carbonate reference sample.
Application
Marine environment research, future prediction of global environment
Relational patent
Item name
:
Density measuring method, calibration reference sample, and method for producing the same
Filing date
:
2018-07-06
Inventor(s)
Katsunori Kimoto