MIRAI MR02-K06 Leg2 Primary Production

Last Modified: 2013-08-23
: MR02-K06 Leg2
: Processed (DMO)-QCed
: JAMSTEC
: POC
:
BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PRIMARY PRODUCTION
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PHOTOSYNTHESIS

For Using Data

Kazuhiko Matsumoto (JAMSTEC)
See Terms and Conditions about constrain of use.
See Terms and Conditions about data citation.
Instrument:
CN mass spectrometer
Primary Production Data during MR02-K06 Leg2 cruise were obtained by the following methods :
- In-situ incubation method (IS)
- Simulated in-situ incubation method (SIS)
Water sampling, incubation, and devices and tracers for analysis for each method are outlined below.
For further information, please see Cruise Report.

1) In-situ incubation (IS) Outline figure
1.1) Vertical sampling : Niskin
1.2) Surface sampling : Bucket
1.3) Sampling layer : 13
1.4) Tracer : NaH13CO3
1.5) Incubation period : 24hours
1.6) Filtration : Whatman GF/F filter was used at dark place.
1.7) Preservation : Filters were kept to freeze at -20degC and dried
in the oven at 45degC.
1.8) Preservation period of frozen filter paper : within 100 days
1.9) Analysis place : YOKOSUKA JAMSTEC, MIRAI
1.10) Analysis device : CN mass spectrometer (see section 3 and 4 for detail)
1.11) Analysis method : Dumas method, Mass spectrometry

2) Simulated in-situ incubation (SIS) Outline figure
2.1) Vertical sampling : Niskin
2.2) Surface sampling : Bucket
2.3) Sampling layer : 3
2.4) Tracer : NaH13CO3
2.5) Incubation period : 24hours
2.6) Filtration : Whatman GF/F filter was used at dark place.
2.7) Preservation : Filters were kept to freeze at -20degC and dried in the oven at 45degC.
2.8) Preservation period of frozen filter paper : within 100 days
2.9) Analysis place : YOKOSUKA JAMSTEC, MIRAI
2.10) Analysis device : CN mass spectrometer (see section 3 and 4 for detail)
2.11) Analysis method : Dumas method, Mass spectrometry

about 2.5) Incubation period of Simulated in situ incubation was 24 hours.
It is mistake though is described in the cruise report as three hours.

 CN mass spectrometer system equipped with R/V Mirai can measure stable isotope
 ratios of 13C and 15N comprised in liquid, solid, and gas states of
 biological or biogenic samples, simultaneously and continuously.
 This system consists of two devices, preprocessing equipment “ROBOPLEP-SL” and
 stable isotope ratio mass spectrometer “EUROPA20-20”.
 
 (1) ROBOPLEP-SL
 A tin capsule containing the sample falls into the combustion tube and is
 converted in the presence of oxygen to CO2, N2, NOx and H2O.
 An elemental copper stage reduces NOx, a MgClO4 trap removes water vapour,
 a switchable Carbosorb trap can be used to remove CO2 (for 15N only analyses)
 and a GC column separates CO2 from N2 (allowing dual isotope analysis).
 And then, it is introduced into the “EUROPA20-20”.
 
 (2) EUROPA20-20
 CO2 and N2 are collided with thermion and ionized in the high vacuum ion source.
 When the generated ions are accelerated by constant voltage and pass through
 the analysis tube, differences in mass (m) and electric charge (z) of isotope
 ions make the different orbits by the magnetic field in the analysis tube.
 Thus, isotopes can be separated by the displacement of the orbits.
 These signals are converted into the frequency at the detector, and transmitted
 to control PC. Blank and drift corrections are conducted on the control software.
 
 see flow diagram. MR02-K06 Leg2_pp_ANCA-SL PDF file
 
 (1) ROBOPLEP-SL
 Manufacturer : SerCon Ltd. (former PDZ Europa Ltd.)
 Instruments :  ANCA-SL ROBOPREP-SL
 S/N :          17001-051
 Sample Range Solids/Liquids : 10 to 1000 μgN, 10 to 1000 μgC.
 Autosampler :  60 position pneumatic autosampler that takes (standard) capsules with up to 47mm in diameter.
 
 (2) EUROPA 20-20
 Manufacturer : SerCon Ltd. (former PDZ Europa Ltd.)
 Instruments :  ANCA-SL EUROPA 20-20
 S/N :          9007-075
 Analyzer and Analysis tube : 120° extended geometry with an 11 cm radius magnetic sector
 Resolution :   m/Δm=95 (N2) 10% valley definition
 Sensitivity :  Inside Vacuum level is 4×10-6mbar in an atmosphere of helium
                20 nmol CO2
                15 nmol N2
 Abundance Sensitivity : Inside Vacuum level is 4×10-6mbar in an atmosphere of helium
                30 ppm for CO2 at 4×10-6mbar in continuous flow mode.
                5 ppm for N2 at 4×10-6mbar in continuous flow mode.
 
 (3) Precision
 All specifications are for n=5 samples.
 It is a natural amount and five time standard deviation of the analysis as for amount 100 μg of the sample.
                13C (0.2 ‰)
                15N (0.5 ‰)
 
 (4) Data processing
 Device control and processing soft : ANCA ver.3.5 (former PDZ Europa Ltd.)
 Fully compatible with Windows 3.1 or Windows 95.
 
 (5) Reference material
 The third-order reference materials whose data values were decided by the second reference materials
 (IAEA-N-1, IAEA-N-2, and IAEA-CH-6) dealt in International Atomic Energy Agency (IAEA) were used.
In this cruise, there is an observation log sheet at the time of the data acquisitions.
If necessary, please contact us from "Contact Us" above.
: MR02-K06 Leg2
: Processed (DMO)-QCed
: JAMSTEC
: POC
:
BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PRIMARY PRODUCTION
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PHOTOSYNTHESIS

Observation Map

Imagery reproduced from ...

Data List

File names
MR02-K06_leg2_pp_IS.csv
MR02-K06_leg2_pp_SIS.csv
: MR02-K06 Leg2
: Processed (DMO)-QCed
: JAMSTEC

PPD IS (MR02-K06 Leg2)

Format information describes column no., column heading mnemonic and comments of In-situ incubation data sheet in MR02-K06 Leg2.
Missing value is presented by -999.

Column No.Column Heading MnemonicComments
1CruiseNOCruiseID
2STNNBRStation number
3CASTNOCast number (refer to CTD cast table of cruise report)
4Inc.TypeIncubation method (IS : in-situ incubation)
5UTC DateCTD start UTC date (refer to CTD cast table of cruise report)
6UTC TimeCTD start UTC time (refer to CTD cast table of cruise report)
7LatitudeCTD start position Latitude degree (refer to CTD cast table of cruise report)
8LongitudeCTD start position Longitude degree (refer to CTD cast table of cruise report)
9BTLNBRBottle identification number
10BTLNBR_FLAGBottle quality flag
11CTD DepthCTD Depth (m)
12CTD PRSCTD Pressure (dbar)
13W-ChlChlorophyll a. quantity (Non-acidification method) (mg/m3)
14H-ChlChlorophyll a. quantity (Acidification method) (mg/m3)
15Inc.DepthIncubation depth (m)
16Inc.TimeIncubation time (hour)
17POC-APOC (Sample A) (µg/L)
18POC-BPOC (Sample B) (µg/L)
1913C-ARatio of 13C (Sample A) (atom%)
2013C-BRatio of 13C (Sample B) (atom%)
21Flag-AFlag of Sample A (for explanation see Quality flags)
22Flag-BFlag of Sample B (for explanation see Quality flags)
23dPOC/d-Adelta POC of Sample A (µgC/L/day)
24dPOC/d-Bdelta POC of Sample B (µgC/L/day)
25AVE of dPOCAverage of dPOC (µgC/L/day)
26Flag-AVEFlag of AVE of dPOC (for explanation see Quality flags)
27Int-PPInitial Primary Production (µgC/day)

about 23 and 24)
The equation to be used in the calculations of dPOC/d-A and dPOC/d-B.
dPOC/d=1.025×POC×(13C-1.084)/{100×(2000×0.01084+200)/(2000+200)-1.084}
1.025 : 13C Stable Isotope discrimination factor
1.084 : 13C ratio of zero time blank POC
100×(2000×0.01084+200)/(2000+200) : Amount of 13C in which 10% of Total dissolved inorganic carbon in seawater was added as tracer.

about 25)
Only the “Flag 1” data in “dPOC/d-A” and “dPOC/d-B” are used for the calculation of “AVE of dPOC”.
(see column No.21 and No.22)

about 27)
The equation to be used in the calculations.
ex.) 0m
Int-PP=(AVE of dPOC at 10m + AVE of dPOC at 0m)×(10m-0m)/2

ex.) 150m
Int-PP=Total Int-PP from 0m to 120m

Literature cited for the equation
Meteorological Agency. 1990. Manuals for oceanographic observation. Japan Weather Association. 253-256pp.

PPD SIS (MR02-K06 Leg2)

Format information describes column no., column heading mnemonic and comments of Simulated in-situ incubation data sheet in MR02-K06 Leg2.
Missing value is presented by -999.

Column No.Column Heading MnemonicComments
1CruiseNOCruiseID
2STNNBRStation number
3CASTNOCast number (refer to CTD cast table of cruise report)
4Inc.TypeIncubation method (SIS : Simulated in-situ incubation)
5UTC DateCTD start UTC date (refer to CTD cast table of cruise report)
6UTC TimeCTD start UTC time (refer to CTD cast table of cruise report)
7LatitudeCTD start position Latitude degree (refer to CTD cast table of cruise report)
8LongitudeCTD start position Longitude degree (refer to CTD cast table of cruise report)
9BTLNBRBottle identification number
10BTLNBR_FLAGBottle quality flag
11CTD DepthCTD Depth (m)
12CTD PRSCTD Pressure (dbar)
13ChlorophyllChlorophyll a. quantity (mg/m3)
14IncubatorOptical transmittance (%)
15Inc.TimeIncubation time (hour)
16POCPOC (µg/L)
1713CRatio of 13C (atom%)
18Carbon uptakedelta POC (µgC/L/day)
19FlagFlag of sample (for explanation see Quality flags)

about 18)
The equation to be used in the calculations.
Carbon uptake=1.025×POC×(13C-1.084)/{100×(2000×0.01084+200)/(2000+200)-1.084}
1.025 : 13C Stable Isotope discrimination factor
1.084 : 13C ratio of zero time blank POC
100×(2000×0.01084+200)/(2000+200) : Amount of 13C in which 10% of Total dissolved inorganic carbon in seawater was added as tracer.

Literature cited for the equation
Meteorological Agency. 1990. Manuals for oceanographic observation. Japan Weather Association. 253-256pp.
: MR02-K06 Leg2
: Processed (DMO)-QCed
: JAMSTEC
Flags of data quality control are as follows.
Quality flags

Related Information

Update History

2013-08-23
An observation data was registerd.
2012-12-25
An observation data was registerd.