MIRAI MR05-01 Bottle Sampling Water Chemical Analysis

 (1) Temperature sensor
     Model:               SBE3, Sea-Bird Electronics, Inc.
     Measurement range:   -5.0 to +35degC
     Accuracy:            0.001degC
     Resolution:          0.0002degC
 (2) Salinity sensor
     Model:               SBE4, Sea-Bird Electronics, Inc.
     Measurement range:   0.0 to 7S/m
     Accuracy:            0.0003S/m
     Resolution:          0.00004S/m
 (3) Pressure sensor
     Model:               SBE9plus, Sea-Bird Electronics, Inc.
     Measurement range:   up to 10500m
     Accuracy:            0.015%F.S.
     Resolution:          0.001%F.S.
 (4) DO sensor
     Model:               SBE43, Sea-Bird Electronics, Inc.
     Measurement range:   0-15ml/l
     Accuracy:            0.1ml/l
     Resolution:          0.01ml/l
 (5) Fluorometer
     Model:               Seapoint Sensors, Inc.
 (6) Transmissometer
     Model:               WET Labs, Inc.
   
     

 1. Dissolved Oxygen
 (1) Instruments: Burette: APB-510 manufactured by Kyoto Electronic Co. Ltd. / 10 cm3 of titration vessel
                 Detector and Software: Automatic photometric titrator manufactured by Kimoto Electronic Co. Ltd
 (2) Methods:     Winkler method/photometric methods
 (3) Precision:   0.087 umol kg-1
 (4) Reference Material/Calibration: 0.001667M KIO3 solution
 

 2. Salinity
 (1) Instruments: Autosal salinometer model 8400B (Guildline Instruments Ltd.)
 (2) Methods:     -
 (3) Precision:   0.0004 PSU
 (4) Reference Material/Calibration: IAPSO Standard Sea Water batch P145 (Ocean Scientific International Ltd.)
 

 3. Silicate
 (1) Instruments: TRAACS800 (Bran+Luebbe)
 (2) Methods:     Molybdenum blue method
 (3) Precision:   C.V. 0.10% (172uM)
 (4) Reference Material/Calibration: RMNS [Aoyama et al., 2007] and Silicate standard solution, the silicate primary standard, is obtained from Kanto Chemical CO., Inc. 
                                    This standard solution is 1000 mg per litter with 0.5 M KOH and prepared for ICP analysis.
 
 4. Nitrate
 (1) Instruments: TRAACS800 (Bran+Luebbe)
 (2) Methods:     Diazotization method (reduced to nitrite by Cd - Cu tube)
 (3) Precision:   C.V. 0.13% (54uM)
 (4) Reference Material/Calibration: KNO3 solution and RMNS [Aoyama et al., 2007]
 

 5. Nitrite
 (1) Instruments: TRAACS800 (Bran+Luebbe)
 (2) Methods:     Diazotization method
 (3) Precision:   C.V. 0.18% (1.2uM)
 (4) Reference Material/Calibration: NaNO2 solution and RMNS [Aoyama et al., 2007]
 

 6. Phosphate
 (1) Instruments: TRAACS800 (Bran+Luebbe)
 (2) Methods:     Molybdenum blue method
 (3) Precision:   C.V. 0.15% (3.7uM)
 (4) Reference Material/Calibration: KH2PO4 solution and RMNS [Aoyama et al., 2007]
 

 7. Total inorganic carbon
 (1) Instruments: the automated TCO2 analyzer (Nippon ANS, Inc.) equipped with carbon coulometer 5012 (UIC Inc.)
 (2) Methods:     coulometry 
 (3) Precision:   1.32umol kg-1 
 (4) Reference Material/Calibration: Na2CO3 solution and the CRM provided by Dr. Dickson in Scripps Institute of Oceanography
 

 8. Total Alkalinity
 (1) Instruments: auto-burette (Radiometer, ABU901), a pH glass electrode (Radiometer, pHG201-7), a reference electrode (Radiometer, REF201)
 (2) Methods:     Modified Gran titration/Closed-cell/potentiometry
 (3) Precision:   1.05 umol kg-1
 (4) Reference Material/Calibration: Na2CO3 solution and the CRM provided by Dr. Dickson in Scripps Institute of Oceanography
 

 9. pH
 (1) Instruments: a glass/reference electrode with a pH/Ion meter (Radiometer PHM95)
 (2) Methods:     potentiometric methods at 25deg-C
 (3) Precision:   0.0012 pH unit
 (4) Reference Material/Calibration: total hydrogen ion scale
 

 10. Chlorophyll-a
 (1) Instruments: Fluorophotometer model 10-AU-005 (Turner designs)
 (2) Methods:     extract in N,N-dimethylformamide/fluorometric determination (acidification method)
 (3) Precision:   -
 (4) Reference Material/Calibration: -