MIRAI MR05-01 Primary Production

Last Modified: 2013-08-23
: MR05-01
: Processed (DMO)-QCed
: JAMSTEC
: POC
:
BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PRIMARY PRODUCTION
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PHOTOSYNTHESIS

For Using Data

Makio Honda (JAMSTEC)
See Terms and Conditions about constrain of use.
See Terms and Conditions about data citation.
Instrument:
CN mass spectrometer
 Primary Production Data during MR05-01 cruise were obtained by the following methods :
- Simulated in-situ incubation method (SIS)
 Water sampling, incubation, and devices and tracers for analysis for each method
are outlined below.
For further information, please see Cruise Report.
  Simulated in-situ incubation (SIS) Outline figure
1)  Vertical sampling : Niskin
2)  Surface sampling :  Bucket
3)  Sampling layer :    8
4)  Tracer :            NaH13CO3
5)  Incubation period : 24hours
6)  Filtration :        Whatman GF/F filter was used at dark place.
7)  Preservation :      Filters were kept to freeze at -20degC and dried
                           in the oven at 45degC.
8)  Preservation period of frozen filter paper : within 30 days
9)  Analysis place :    MIRAI
10) Analysis device :   CN mass spectrometer (see section 3 and 4 for detail)
11) Analysis method :   Dumas method, Mass spectrometry
 CN mass spectrometer system equipped with R/V Mirai can measure stable isotope
ratios of 13C and 15N comprised in liquid, solid, and gas states of
biological or biogenic samples, simultaneously and continuously.
This system consists of two devices, preprocessing equipment “ROBOPLEP-SL” and
stable isotope ratio mass spectrometer “EUROPA20-20”.

(1) ROBOPLEP-SL
A tin capsule containing the sample falls into the combustion tube and is
converted in the presence of oxygen to CO2, N2, NOx and H2O.
An elemental copper stage reduces NOx, a MgClO4 trap removes water vapour,
a switchable Carbosorb trap can be used to remove CO2 (for 15N only analyses)
and a GC column separates CO2 from N2 (allowing dual isotope analysis).
And then, it is introduced into the “EUROPA20-20”.

(2) EUROPA20-20
CO2 and N2 are collided with thermion and ionized in the high vacuum ion source.
When the generated ions are accelerated by constant voltage and pass through
the analysis tube, differences in mass (m) and electric charge (z) of isotope
ions make the different orbits by the magnetic field in the analysis tube.
Thus, isotopes can be separated by the displacement of the orbits.
These signals are converted into the frequency at the detector, and transmitted
to control PC. Blank and drift corrections are conducted on the control software.

see flow diagram. MR05-01_pp_ANCA-SL PDF file
(1) ROBOPLEP-SL
Manufacturer : SerCon Ltd. (former PDZ Europa Ltd.)
Instruments :  ANCA-SL ROBOPREP-SL
S/N :          17001-051
Sample Range Solids/Liquids : 10 to 1000 µgN, 10 to 1000 µgC.
Autosampler :  60 position pneumatic autosampler that takes (standard) capsules with up to 47mm in diameter.

(2) EUROPA 20-20
Manufacturer : SerCon Ltd. (former PDZ Europa Ltd.)
Instruments :  ANCA-SL EUROPA 20-20
S/N :          9007-075
Analyzer and Analysis tube : 120° extended geometry with an 11 cm radius magnetic sector
Resolution :   m/Δm=95 (N2) 10% valley definition
Sensitivity :  Inside Vacuum level is 4×10-6mbar in an atmosphere of helium
                20 nmol CO2
                15 nmol N2
Abundance Sensitivity : Inside Vacuum level is 4×10-6mbar in an atmosphere of helium
                30 ppm for CO2 at 4×10-6mbar in continuous flow mode.
                5 ppm for N2 at 4×10-6mbar in continuous flow mode.

(3) Precision
All specifications are for n=5 samples.
It is a natural amount and five time standard deviation of the analysis as for amount 100 µg of the sample.
                13C (0.2 ‰)
                15N (0.5 ‰)

(4) Data processing
Device control and processing soft : ANCA ver.3.5 (former PDZ Europa Ltd.)
Fully compatible with Windows 3.1 or Windows 95.

(5) Reference material
The third-order reference materials whose data values were decided by the second reference materials
(IAEA-N-1, IAEA-N-2, and IAEA-CH-6) dealt in International Atomic Energy Agency (IAEA) were used.
: MR05-01
: Processed (DMO)-QCed
: JAMSTEC
: POC
:
BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PRIMARY PRODUCTION
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PHOTOSYNTHESIS

Observation Map

Imagery reproduced from ...

Data List

  File names
MR05-01_pp_SIS.csv
: MR05-01
: Processed (DMO)-QCed
: JAMSTEC

PPD SIS (MR05-01)

Format information describes column no., column heading mnemonic and comments of In-situ incubation data sheet in MR05-01. Missing value is presented by -999.


Column No.Column Heading MnemonicComments
1CruiseNOCruiseID
2STNNBRStation number
3CASTNOCast number (refer to CTD cast table of cruise report)
4Inc.TypeIncubation method (IS : in-situ incubation)
5UTC DateCTD start UTC date (refer to CTD cast table of cruise report)
6UTC TimeCTD start UTC time (refer to CTD cast table of cruise report)
7LatitudeCTD start position Latitude degree (refer to CTD cast table of cruise report)
8LongitudeCTD start position Longitude degree (refer to CTD cast table of cruise report)
9BTLNBRBottle identification number
10BTLNBR_FLAGBottle quality flag
11CTD DepthCTD Depth (m)
12CTD PRSCTD Pressure (dbar)
13ChlorophyllChlorophyll a. quantity (Welschmeyer method) (µg/L)
14Light intensityOptical transmittance (%)
15Inc.TimeIncubation time (hour)
16POC-APOC (Sample A) (µg/L)
17POC-BPOC (Sample B) (µg/L)
1813C-ARatio of 13C (Sample A) (atom%)
1913C-BRatio of 13C (Sample B) (atom%)
20Flag-AFlag of Sample A (for explanation see Quality flags)
21Flag-BFlag of Sample B (for explanation see Quality flags)
22dPOC-Adelta POC of Sample A (µg/L/day)
23dPOC-Bdelta POC of Sample B (µg/L/day)
24AVE of dPOCAverage of dPOC (µg/L/day)
25Flag-AVEFlag of AVE of dPOC (for explanation see Quality flags)
26PPPrimary productivity (mg/m2/day)
27d POC-A/tdelta POC of Sample A per hour (µg/L/h)
28d POC-B/tdelta POC of Sample B per hour (µg/L/h)
29PB-Ad POC-A/t/Chl.a (mgC/mg chl.a/h)
30PB-Bd POC-B/t/Chl.a (mgC/mg chl.a/h)
31RemarksFlag explanation etc.

about 22, 23, 26, 27, 28, 29 and 30)
For calculations, please see Cruise Report.

about 24)
Only the “Flag 1” data in “dPOC-A” and “dPOC-B” are used for the calculation of “AVE of dPOC”.
(see column No.20 and No.21)

: MR05-01
: Processed (DMO)-QCed
: JAMSTEC
Flags of data quality control are as follows.

Related Information

Update History

2013-08-23
An observation data was registerd.
2012-11-25
An observation data was registerd.