MIRAI MR08-05 Phytoplankton Biomass

Last Modified: 2012-10-26
: MR08-05
: Processed (DMO)-QCed
: JAMSTEC
: Cell number and cell size of phytoplankton, and their composition
:
BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON

For Using Data

Kazuhiko Matsumoto (JAMSTEC)
See Terms and Conditions about constrain of use.
See Terms and Conditions about data citation.
Instrument:
Absorption spectro-photometer
Phytoplankton biomass data by Flow cytometry during MR08-05 cruise were obtained by the following methods. Water sampling, filtration, and devices and standard materials for analysis for this method are outlined below. For further information, please see Cruise Report.
1) Vertical sampling: Niskin
2) Surface sampling: Bucket
3) Sampling layer: 8
4) Filter and filtration: Nuclepore filter 3µm pore size
5) Sample fixed: Glutaraldehyde(1%)
6) Store condition : dark at 4degC
7) Stored time : within 24 hours
8) Analysis place: R/V MIRAI
9) Analysis device: Flow cytometry system
Flow cytometry system measures forward light scatter (FS) and fluorescence from cells in a sample fluid (i.e., sheath liquid and sample materials) at the same time, by irradiating lights from equipped xenon arc lamp as illumination source at the center of the flow cell of the sample fluid.
Phytoplankton cell number and cell size are estimated by the FS signals.
The emitted fluorescence signals are divided by 565-605nm and >615nm, by which phytoplankton is classified.
Consequently, cell number and cell size of phytoplankton and their composition in the ocean are estimated.

Measurement principle of Flow cytometer is as below:
Flow system: makes flow of the cells by using sheath fluid. Optical detection system: measures FS and emitted fluorescence from cells by irradiating lights at the center of the flow cell of the sample fluid. Electric pulse processing system: evaluates a detected electric pulse by a circuit. Data processing system: makes various histograms and analyzes data.
Manufacturer: Bio-Rad Laboratories Inc.
Instruments type: BRYTE HS system
Light source : 75W Xenon arc lamp
Excitation wavelength : 350-650nm
Detector : high-performance PMT (Photo multiplier tube)
Analyzed volume : 75µL
Flow rate : 10µLmin-1
Sheath fluid: Milli-Q water
Filter block : B2 (excitation filter)
and OR1 (fluorescence separator)
B2 : Excitation filter 390-490nm
Beam-splitter 510nm
Emission filter 515-720nm
OR1 : Emission filter1 565-605nm (orange)
Beam-splitter 600nm
Emission filter2 >615nm (red)
Device control and processing soft : WinBryte
Standard beads (Polysciences Inc.) 2.764µm
Kazuhiko Matsumoto, Ken Furuya and Takeshi Kawano. (2004)
Association of picophytoplankton distribution with ENSO events
in the equatorial Pacific between 145°E and 160°W. Deep-Sea Res. I 51(2004) 1851-1871.
: MR08-05
: Processed (DMO)-QCed
: JAMSTEC
: Cell number and cell size of phytoplankton, and their composition
:
BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON

Observation Map

Imagery reproduced from ...

Data List

  File names
MR08-05_FC.csv
: MR08-05
: Processed (DMO)-QCed
: JAMSTEC

Phytoplankton Biomass Data Sheet Format

Format information describes column no., column heading mnemonic and comments of phytoplankton biomass data sheet by Flow cytometry in MR08-05.
Missing value is presented by -999.

Column No.Column Heading MnemonicComments
1CruiseIDCruiseID
2STNNBRStation number (refer to CTD cast table of cruise report)
3CASTNOCast number (refer to CTD cast table of cruise report)
4UTC DateCTD start UTC date (refer to CTD cast table of cruise report)
5UTC TimeCTD start UTC time (refer to CTD cast table of cruise report)
6LatitudeCTD start position Latitude north degree (refer to CTD cast table of cruise report)
7LongitudeCTD start position Longitude east degree (refer to CTD cast table of cruise report)
8BTLNBRBottle identification number
9BTLNBR_FLAG_WBottle quality flag (for explanation see Quality flags)
10CTD DepthCTD Depth (m)
11CTD PRSCTD Pressure (dbar)
12CTDPRS_FLAG_WCTD Pressure flag (for explanation see Quality flags)
13SynechococcusCell count (×1000 cells/ml)
14Picoeukaryotes-1Cell count (×1000 cells/ml)
15Picoeukaryotes-2Cell count (×1000 cells/ml)
16Picoeukaryotes-3Cell count (×1000 cells/ml)
17RemarksStation name
: MR08-05
: Processed (DMO)-QCed
: JAMSTEC
Flags of data quality control are as follows.

Related Information

Update History

2012-10-26
An observation data was registerd.