MIRAI MR08-05 Photosynthetic Pigments

Last Modified: 2018-09-13
: MR08-05
: Processed (DMO)-QCed
: JAMSTEC
: photosynthetic pigments
:
OCEANS > OCEAN CHEMISTRY > CHLOROPHYLL
BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PHOTOSYNTHESIS

For Using Data

PI (Principal Investigator) : Kazuhiko Matsumoto (JAMSTEC)
See Terms and Conditions about constrain of use.
See Terms and Conditions about data citation.
Instrument:
High-performance liquid chromatography ( - MR10-03 Leg2)
Photosynthetic pigments data by HPLC during MR08-05 cruise were obtained by the following methods.
Water sampling, filtration, and devices and standard materials for analysis for this method are outlined below.
For further information, please see Cruise Report.
1) Vertical sampling                          : Niskin
2) Surface sampling                           : Bucket
3) Sampling layer:                            : 8
4) Filter and filtration                      : Whatman GF/F 47mm was used at dark place.
5) Freezing and drying                        : -20degC, 5 hours
6) Extract reagent                            : N,N-dimethylformamide (HPLC-grade)
7) Extract time                               : -20degC, 24 hours
8) Preservation period of frozen filter paper : a few days
9) Analysis place                             : MIRAI
10) Analysis device                           : High-performance liquid chromatography (HPLC)
11) Stationary phase                          : YMC C8 column, 150×4.6mm, 25degC
12) Mobile phase (unit in volume ratio):
Eluant A:  methanol : acetonitrile : aqueous pyridin (0.25M pyridine) = 50 : 25 : 25
Eluant B:  acetonitrile : acetone = 80 : 20
13) Analysis pigment number                   : 26 pigments (see cruise report)

High-performance liquid chromatography (HPLC) is a kind of column chromatography to separate, identify,
and quantify individual chemical compounds from mixtures of compounds by a difference of chemical attractions with
the column's stationary phase.
High pressure to propels the mobile phase allow for a better separation resolution and sensitivity
than ordinary column chromatography.

In MIRAI, HPLC are used for separating and quantifying various phytoplankton pigments in natural seawater.
Taxonomic composition of phytoplankton can be estimated by measuring composition of their pigments.
In this cruise, reversed phase C8 Columns and pyridine are used as stationary and mobile phases, respectively,
based on a method of Zapata et al. (2000). More pigments can be separated by using C8 column than C18 column
which was conventionally used. Pyridine used as a mobile phase is suitable for a better separation of phytoplankton
ingradients such as carotenoids and chlorophils. HPLC consists of pump, pump controller, auto-sampler, PDA detector,
Fluorescence detector, column heater, control PC and printer.

Please refer to the following for the device whole view.
PDF file
Manufacturer:      Waters co.
Instruments type:  Waters 616 (pump)
                   Waters 600S (pump controller)
                   Waters 717plus (auto-sampler)
                   Waters 996 (photodiode array detector)
                   Waters 474 (Fluorescence detector)
                   Waters N62009 (column heater)
                   COMPAC DESKPRO (control PC)

Pigment detection and identification:
Divinyl Chlorophyll a, Chlorophyll a            : 661.4nm-absorption maximum in red band
Chlorophyllide a, Pheophorbide a, Pheophytin a  : 663.9nm-absorption maximum in red band
Chlorophyll b                                   : 457.2nm-absorption maximum in red band
[3,8-Divinyl]-Protochlorophyllide               : 440.0nm-absorption maximum in red band
Others                                          : 460.0nm-absorption maximum in red band
Device control and processing soft: Millennium32 (Waters Co. Ltd.)
Fully compatible with Windows 98 Second edition

Chlorophyll a : Sigma-Aldrich Co.
Chlorophyll b : Sigma-Aldrich Co.
Other 23 pigments : DHI co.
trans-β-Apo-8'-carotenal (Internal standard) : Sigma-Aldrich Co.
Zapata, M., Rodriguez, F. and Garrido, J. L. (2000)
Separation of chlorophylls and carotenoids from marine phytoplankton: a new HPLC meted using a reversed
phase C8 column and pyridine-containing mobile phases. Mar. Ecol. Prog. Ser., 195, 29-45.
There are some description error for photosynthetic pigment data of this cruise.
Please refer to the errata of the cruise report.
: MR08-05
: Processed (DMO)-QCed
: JAMSTEC
: photosynthetic pigments
:
OCEANS > OCEAN CHEMISTRY > CHLOROPHYLL
BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PHOTOSYNTHESIS

Observation Map

Imagery reproduced from ...

Data List

  File names
MR08-05_HPLC.csv
: MR08-05
: Processed (DMO)-QCed
: JAMSTEC

photosynthetic pigments Data Sheet Format

    Format information describes column no., column heading mnemonic and comments of photosynthetic pigments
    data sheet by HPLC in MR08-05.
    Missing value is presented by -9.

    Column No.Column Heading MnemonicComments
    1CruiseIDCruiseID
    2STNNBRStation number (refer to CTD cast table of cruise report)
    3CASTNOCast number (refer to CTD cast table of cruise report)
    4UTC DateCTD start UTC date (refer to CTD cast table of cruise report)
    5UTC TimeCTD start UTC time (refer to CTD cast table of cruise report)
    6LatitudeCTD start position Latitude north degree (refer to CTD cast table of cruise report)
    7LongitudeCTD start position Longitude east degree (refer to CTD cast table of cruise report)
    8BTLNBRBottle identification number
    9BTLNBR_FLAG_WBottle quality flag (for explanation see Quality flags)
    10CTD DepthCTD Depth (m)
    11CTD PRSCTD Pressure (dbar)
    12CTDPRS_FLAG_WCTD Pressure flag (for explanation see Quality flags)
    13CHLHPLCChlorophyll a quantity (mg/m3)
    14CHLHPLC_FLAG_WChlorophyll a quantity flag (for explanation see Quality flags)
    15CHLC3Chlorophyll c3 quantity (mg/m3)
    16CHLC3_FLAG_WChlorophyll c3 quantity flag (for explanation see Quality flags)
    17CHLIDEAChlorophyllide a quantity (mg/m3)
    18CHLIDEA_FLAG_WChlorophyllide a quantity flag (for explanation see Quality flags)
    19MGDVP[3,8-Divinyl]-Protochlorophyllide quantity (mg/m3)
    20MGDVP_FLAG_W[3,8-Divinyl]-Protochlorophyllide quantity flag (for explanation see Quality flags)
    21CHLC2Chlorophyll c2 quantity (mg/m3)
    22CHLC2_FLAG_WChlorophyll c2 quantity flag (for explanation see Quality flags)
    23PERIDPeridinin quantity (mg/m3)
    24PERID_FLAG_WPeridinin quantity flag (for explanation see Quality flags)
    25PHIDEAPheophorbide a quantity (mg/m3)
    26PHIDEA_FLAG_WPheophorbide a quantity flag (for explanation see Quality flags)
    27BUTFUCO19'-butanoyloxyfucoxanthin quantity (mg/m3)
    28BUTFUCO_FLAG_W19'-butanoyloxyfucoxanthin quantity flag (for explanation see Quality flags)
    29FUCOFucoxanthin quantity (mg/m3)
    30FUCO_FLAG_WFucoxanthin quantity flag (for explanation see Quality flags)
    31NEONeoxanthin quantity (mg/m3)
    32NEO_FLAG_WNeoxanthin quantity flag (for explanation see Quality flags)
    33PRASPrasinoxanthin quantity (mg/m3)
    34PRAS_FLAG_WPrasinoxanthin quantity flag (for explanation see Quality flags)
    35HEXFUCO19'-hexanoyloxyfucoxanthin quantity (mg/m3)
    36HEXFUCO_FLAG_W19'-hexanoyloxyfucoxanthin quantity flag (for explanation see Quality flags)
    37VIOLAViolaxanthin quantity (mg/m3)
    38VIOLA_FLAG_WViolaxanthin quantity flag (for explanation see Quality flags)
    39DIADINODiadinoxanthin quantity (mg/m3)
    40DIADINO_FLAG_WDiadinoxanthin uantity flag (for explanation see Quality flags)
    41ANTHAntheraxanthin quantity (mg/m3)
    42ANTH_FLAG_WAntheraxanthin quantity flag (for explanation see Quality flags)
    43ALLOAlloxanthin quantity (mg/m3)
    44ALLO_FLAG_WAlloxanthin quantity flag (for explanation see Quality flags)
    45DIATODiatoxanthin quantity (mg/m3)
    46DIATO_FLAG_WDiatoxanthin quantity flag (for explanation see Quality flags)
    47ZEAZeaxanthin quantity (mg/m3)
    48ZEA_FLAG_WZeaxanthin quantity flag (for explanation see Quality flags)
    49LUTLutein quantity (mg/m3)
    50LUT_FLAG_WLutein quantity flag (for explanation see Quality flags)
    51CROCOCrocoxanthin quantity (mg/m3)
    52CROCO_FLAG_WCrocoxanthin quantity flag (for explanation see Quality flags)
    53CHLBChlorophyll b quantity (mg/m3)
    54CHLB_FLAG_WChlorophyll b quantity flag (for explanation see Quality flags)
    55DCHLADivinyl Chlorophyll a quantity (mg/m3)
    56DCHLA_FLAG_WDivinyl Chlorophyll a quantity flag (for explanation see Quality flags)
    57PHYTINAPheophytin a quantity (mg/m3)
    58PHYTINA_FLAG_WPheophytin a quantity flag (for explanation see Quality flags)
    59ALPHACAlpha-carotene quantity (mg/m3)
    60ALPHAC_FLAG_WAlpha-carotene quantity flag (for explanation see Quality flags)
    61BETACBeta-carotene quantity (mg/m3)
    62BETAC_FLAG_WBeta-carotene quantity flag (for explanation see Quality flags)
    63RemarksStation name
: MR08-05
: Processed (DMO)-QCed
: JAMSTEC
Flags of data quality control are as follows.

Related Information

Update History

2018-09-13
An observation data was registerd.
2013-08-29
An observation data was registerd.
2012-10-26
An observation data was registerd.