BIOSPHERE | > AQUATIC ECOSYSTEMS | > PLANKTON | > PHYTOPLANKTON |
BIOSPHERE | > ECOLOGICAL DYNAMICS | > ECOSYSTEM FUNCTIONS | > PRIMARY PRODUCTION |
BIOSPHERE | > ECOLOGICAL DYNAMICS | > ECOSYSTEM FUNCTIONS | > PHOTOSYNTHESIS |
Primary Production Data during MR08-05 cruise were obtained by the following methods : - In-situ incubation method (IS) - Simulated in-situ incubation method (SIS) Water sampling, incubation, and devices and tracers for analysis for each method are outlined below. For further information, please see Cruise Report.
1) In-situ incubation (IS) Outline figure 1.1) Vertical sampling : Niskin 1.2) Surface sampling : Bucket 1.3) Sampling layer : 8 1.4) Tracer : NaH13CO3, K15NO3 1.5) Incubation period : 24hours 1.6) Filtration : Whatman GF/F filter was used at dark place. 1.7) Preservation : Filters were kept to freeze at -20degC and dried in the oven at 45degC. 1.8) Preservation period of frozen filter paper : within 30 days 1.9) Analysis place : MIRAI 1.10) Analysis device : CN mass spectrometer 1.11) Analysis method : Dumas method, Mass spectrometry 2) Simulated in-situ incubation (SIS) Outline figure 2.1) Vertical sampling : Niskin 2.2) Surface sampling : Bucket 2.3) Sampling layer : 8 2.4) Tracer : NaH13CO3, K15NO3 2.5) Incubation period : 24hours 2.6) Filtration : Whatman GF/F filter was used at dark place. 2.7) Preservation : Filters were kept to freeze at -20degC and dried in the oven at 45degC. 2.8) Preservation period of frozen filter paper : within 30 days 2.9) Analysis place : MIRAI 2.10) Analysis device : CN mass spectrometer 2.11) Analysis method : Dumas method, Mass spectrometry
CN mass spectrometer system equipped with R/V Mirai can measure stable isotope ratios of 13C and 15N comprised in liquid, solid, and gas states of biological or biogenic samples, simultaneously and continuously. This system consists of two devices, preprocessing equipment “ROBOPLEP-SL” and stable isotope ratio mass spectrometer “EUROPA20-20”. (1) ROBOPLEP-SL A tin capsule containing the sample falls into the combustion tube and is converted in the presence of oxygen to CO2, N2, NOx and H2O. An elemental copper stage reduces NOx, a MgClO4 trap removes water vapour, a switchable Carbosorb trap can be used to remove CO2 (for 15N only analyses) and a GC column separates CO2 from N2 (allowing dual isotope analysis). And then, it is introduced into the “EUROPA20-20”. (2) EUROPA20-20 CO2 and N2 are collided with thermion and ionized in the high vacuum ion source. When the generated ions are accelerated by constant voltage and pass through the analysis tube, differences in mass (m) and electric charge (z) of isotope ions make the different orbits by the magnetic field in the analysis tube. Thus, isotopes can be separated by the displacement of the orbits. These signals are converted into the frequency at the detector, and transmitted to control PC. Blank and drift corrections are conducted on the control software. see flow diagram. MR08-05_pp_ANCA-SL PDF file
(1) ROBOPLEP-SL Manufacturer : SerCon Ltd. (former PDZ Europa Ltd.) Instruments : ANCA-SL ROBOPREP-SL S/N : 17001-051 Sample Range Solids/Liquids : 10 to 1000 µgN, 10 to 1000 µgC. Autosampler : 60 position pneumatic autosampler that takes (standard) capsules with up to 47mm in diameter. (2) EUROPA 20-20 Manufacturer : SerCon Ltd. (former PDZ Europa Ltd.) Instruments : ANCA-SL EUROPA 20-20 S/N : 9007-075 Analyzer and Analysis tube : 120° extended geometry with an 11 cm radius magnetic sector Resolution : m/Δm=95 (N2) 10% valley definition Sensitivity : Inside Vacuum level is 4×10-6mbar in an atmosphere of helium 20 nmol CO2 15 nmol N2 Abundance Sensitivity : Inside Vacuum level is 4×10-6mbar in an atmosphere of helium 30 ppm for CO2 at 4×10-6mbar in continuous flow mode. 5 ppm for N2 at 4×10-6mbar in continuous flow mode. (3) Precision All specifications are for n=5 samples. It is a natural amount and five time standard deviation of the analysis as for amount 100 µg of the sample. 13C (0.2 ‰) 15N (0.5 ‰) (4) Data processing Device control and processing soft : ANCA ver.3.5 (former PDZ Europa Ltd.) Fully compatible with Windows 3.1 or Windows 95. (5) Reference material The third-order reference materials whose data values were decided by the second reference materials (IAEA-N-1, IAEA-N-2, and IAEA-CH-6) dealt in International Atomic Energy Agency (IAEA) were used.
BIOSPHERE | > AQUATIC ECOSYSTEMS | > PLANKTON | > PHYTOPLANKTON |
BIOSPHERE | > ECOLOGICAL DYNAMICS | > ECOSYSTEM FUNCTIONS | > PRIMARY PRODUCTION |
BIOSPHERE | > ECOLOGICAL DYNAMICS | > ECOSYSTEM FUNCTIONS | > PHOTOSYNTHESIS |
Column No. | Column Heading Mnemonic | Comments |
---|---|---|
1 | CruiseID | CruiseID |
2 | STNNBR | Station name |
3 | CASTNO | Cast number (refer to CTD cast table of cruise report) |
4 | Inc.Type | Incubation method (IS : in-situ incubation) |
5 | UTC Date | CTD start UTC date (refer to CTD cast table of cruise report) |
6 | UTC Time | CTD start UTC time (refer to CTD cast table of cruise report) |
7 | Latitude | CTD start position Latitude north degree (refer to CTD cast table of cruise report) |
8 | Longitude | CTD start position Longitude east degree (refer to CTD cast table of cruise report) |
9 | BTLNBR | Bottle identification number |
10 | BTLNBR_FLAG | Bottle quality flag (for explanation see Quality flags) |
11 | CTD Depth | CTD Depth (m) |
12 | CTD PRS | CTD Pressure (dbar) |
13 | Light intensity | Optical transmittance (%) |
14 | Inc.Time | Incubation time (hour) |
15 | Spike 13C | Spiked 13C solution (µM) |
16 | Filt. Vol | Filtering volume (mL) |
17 | POC | POC (µg/L) |
18 | POC_FLAG | Flag of POC (for explanation see Quality flags) |
19 | 13C | Ratio of 13C (atom%) |
20 | 13C_FLAG | Flag of 13C (for explanation see Quality flags) |
21 | DIC | Total dissolved inorganic carbon (µmol/kg) |
22 | Salinity | Salinity (PSU) |
23 | Chl.a | Chlorophyll a. quantity (µg/L) |
24 | Density | Seawater density (kg/L) |
25 | DIC | Total dissolved inorganic carbon (µmol/L) |
26 | POC | POC (mg/m3) |
27 | 13C0 | Particulate organic carbon (13C) before incubation (atom%) |
28 | 13Cxs | Ratio of 13C (atom%) - 13C0 (atom%) |
29 | 13Csw | Concentration of 13C of ambient seawater with a tracer (%) |
30 | d-POC | Delta POC/hour (mgC/m3/h) |
31 | PP Ave | Average of delta POC/day (mgC/m3/day) |
32 | Integrated PP | Initial Primary Production (mgC/m2/day) |
33 | PB | d-POC/Chl.a (mgC/mg chl.a/h) |
34 | Remarks | Station number (refer to CTD cast table of cruise report) |
Column No. | Column Heading Mnemonic | Comments |
---|---|---|
1 | CruiseID | CruiseID |
2 | STNNBR | Station name |
3 | CASTNO | Cast number (refer to CTD cast table of cruise report) |
4 | Inc.Type | Incubation method (SIS : simulated in-situ incubation) |
5 | UTC Date | CTD start UTC date (refer to CTD cast table of cruise report) |
6 | UTC Time | CTD start UTC time (refer to CTD cast table of cruise report) |
7 | Latitude | CTD start position Latitude north degree (refer to CTD cast table of cruise report) |
8 | Longitude | CTD start position Longitude east degree (refer to CTD cast table of cruise report) |
9 | BTLNBR | Bottle identification number |
10 | BTLNBR_FLAG | Bottle quality flag (for explanation see Quality flags) |
11 | CTD Depth | CTD Depth (m) |
12 | CTD PRS | CTD Pressure (dbar) |
13 | Light intensity | Optical transmittance (%) |
14 | Inc.Time | Incubation time (hour) |
15 | Spike 13C | Spiked 13C solution (µM) |
16 | Filt. Vol | Filtering volume (mL) |
17 | POC | POC (µg/L) |
18 | POC_FLAG | Flag of POC (for explanation see Quality flags) |
19 | 13C | Ratio of 13C (atom%) |
20 | 13C_FLAG | Flag of 13C (for explanation see Quality flags) |
21 | DIC | Total dissolved inorganic carbon (µmol/kg) |
22 | Salinity | Salinity (PSU) |
23 | Chl.a | Chlorophyll a. quantity (µg/L) |
24 | Density | Seawater density (kg/L) |
25 | DIC | Total dissolved inorganic carbon (µmol/L) |
26 | POC | POC (mg/m3) |
27 | 13C0 | Particulate organic carbon(13C) before incubation (atom%) |
28 | 13Cxs | Ratio of 13C - 13C0 (atom%) |
29 | 13Csw | Concentration of 13C of ambient seawater with a tracer(%) |
30 | d-POC | Delta POC/hour (mgC/m3/h) |
31 | PP Ave | Average of delta POC/day (mgC/m3/day) |
32 | Integrated PP | Initial Primary Production (mgC/m2/day) |
33 | PB | d-POC/Chl.a (mgC/mg chl.a/h) |
34 | Remarks | Station number (refer to CTD cast table of cruise report) |