MIRAI MR08-05 Primary Production

Last Modified: 2013-08-29
: MR08-05
: Processed (DMO)-QCed
: JAMSTEC
: POC
:
BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PRIMARY PRODUCTION
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PHOTOSYNTHESIS

For Using Data

Makio Honda (JAMSTEC)
See Terms and Conditions about constrain of use.
See Terms and Conditions about data citation.
Instrument:
CN mass spectrometer
 Primary Production Data during MR08-05 cruise were obtained by the following methods :
 - In-situ incubation method (IS)
 - Simulated in-situ incubation method (SIS)
 Water sampling, incubation, and devices and tracers for analysis for each method
 are outlined below.
 For further information, please see Cruise Report.
 
 1) In-situ incubation (IS) Outline figure
 1.1)  Vertical sampling : Niskin
 1.2)  Surface sampling :  Bucket
 1.3)  Sampling layer :    8
 1.4)  Tracer :            NaH13CO3, K15NO3
 1.5)  Incubation period : 24hours
 1.6)  Filtration :        Whatman GF/F filter was used at dark place.
 1.7)  Preservation :      Filters were kept to freeze at -20degC and dried
                           in the oven at 45degC.
 1.8)  Preservation period of frozen filter paper : within 30 days
 1.9)  Analysis place :    MIRAI
 1.10) Analysis device :   CN mass spectrometer
 1.11) Analysis method :   Dumas method, Mass spectrometry
 
 2) Simulated in-situ incubation (SIS) Outline figure
 2.1)  Vertical sampling : Niskin
 2.2)  Surface sampling :  Bucket
 2.3)  Sampling layer :    8
 2.4)  Tracer :            NaH13CO3, K15NO3
 2.5)  Incubation period : 24hours
 2.6)  Filtration :        Whatman GF/F filter was used at dark place.
 2.7)  Preservation :      Filters were kept to freeze at -20degC and dried
                           in the oven at 45degC.
 2.8)  Preservation period of frozen filter paper : within 30 days
 2.9)  Analysis place :    MIRAI
 2.10) Analysis device :   CN mass spectrometer
 2.11) Analysis method :   Dumas method, Mass spectrometry
 
 CN mass spectrometer system equipped with R/V Mirai can measure stable isotope
 ratios of 13C and 15N comprised in liquid, solid, and gas states of
 biological or biogenic samples, simultaneously and continuously.
 This system consists of two devices, preprocessing equipment “ROBOPLEP-SL” and
 stable isotope ratio mass spectrometer “EUROPA20-20”.
 
 (1) ROBOPLEP-SL
 A tin capsule containing the sample falls into the combustion tube and is
 converted in the presence of oxygen to CO2, N2, NOx and H2O.
 An elemental copper stage reduces NOx, a MgClO4 trap removes water vapour,
 a switchable Carbosorb trap can be used to remove CO2 (for 15N only analyses)
 and a GC column separates CO2 from N2 (allowing dual isotope analysis).
 And then, it is introduced into the “EUROPA20-20”.
 
 (2) EUROPA20-20
 CO2 and N2 are collided with thermion and ionized in the high vacuum ion source.
 When the generated ions are accelerated by constant voltage and pass through
 the analysis tube, differences in mass (m) and electric charge (z) of isotope
 ions make the different orbits by the magnetic field in the analysis tube.
 Thus, isotopes can be separated by the displacement of the orbits.
 These signals are converted into the frequency at the detector, and transmitted
 to control PC. Blank and drift corrections are conducted on the control software.
 
 see flow diagram. MR08-05_pp_ANCA-SL PDF file
 

 (1) ROBOPLEP-SL
 Manufacturer : SerCon Ltd. (former PDZ Europa Ltd.)
 Instruments :  ANCA-SL ROBOPREP-SL
 S/N :          17001-051
 Sample Range Solids/Liquids : 10 to 1000 µgN, 10 to 1000 µgC.
 Autosampler :  60 position pneumatic autosampler that takes (standard) capsules with up to 47mm in diameter.
 
 (2) EUROPA 20-20
 Manufacturer : SerCon Ltd. (former PDZ Europa Ltd.)
 Instruments :  ANCA-SL EUROPA 20-20
 S/N :          9007-075
 Analyzer and Analysis tube : 120° extended geometry with an 11 cm radius magnetic sector
 Resolution :   m/Δm=95 (N2) 10% valley definition
 Sensitivity :  Inside Vacuum level is 4×10-6mbar in an atmosphere of helium
                20 nmol CO2
                15 nmol N2
 Abundance Sensitivity : Inside Vacuum level is 4×10-6mbar in an atmosphere of helium
                30 ppm for CO2 at 4×10-6mbar in continuous flow mode.
                5 ppm for N2 at 4×10-6mbar in continuous flow mode.
 
 (3) Precision
 All specifications are for n=5 samples.
 It is a natural amount and five time standard deviation of the analysis as for amount 100 µg of the sample.
                13C (0.2 ‰)
                15N (0.5 ‰)
 
 (4) Data processing
 Device control and processing soft : ANCA ver.3.5 (former PDZ Europa Ltd.)
 Fully compatible with Windows 3.1 or Windows 95.
 
 (5) Reference material
 The third-order reference materials whose data values were decided by the second reference materials
 (IAEA-N-1, IAEA-N-2, and IAEA-CH-6) dealt in International Atomic Energy Agency (IAEA) were used.

In this cruise, there is an observation log sheet at the time of the data acquisitions.
If necessary, please contact us from "Contact Us" above.
: MR08-05
: Processed (DMO)-QCed
: JAMSTEC
: POC
:
BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PRIMARY PRODUCTION
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PHOTOSYNTHESIS

Observation Map

Imagery reproduced from ...

Data List

File names
MR08-05_pp_IS.csv
MR08-05_pp_SIS.csv
: MR08-05
: Processed (DMO)-QCed
: JAMSTEC

Primary Productivity Data Sheet Format (IS : in-situ incubation)

Format information describes column no., column heading mnemonic and comments of in-situ incubation primary productivity data sheet in MR08-05.
Missing value is presented by -999.

Column No.Column Heading MnemonicComments
1CruiseIDCruiseID
2STNNBRStation name
3CASTNOCast number (refer to CTD cast table of cruise report)
4Inc.TypeIncubation method (IS : in-situ incubation)
5UTC DateCTD start UTC date (refer to CTD cast table of cruise report)
6UTC TimeCTD start UTC time (refer to CTD cast table of cruise report)
7LatitudeCTD start position Latitude north degree (refer to CTD cast table of cruise report)
8LongitudeCTD start position Longitude east degree (refer to CTD cast table of cruise report)
9BTLNBRBottle identification number
10BTLNBR_FLAGBottle quality flag (for explanation see Quality flags)
11CTD DepthCTD Depth (m)
12CTD PRSCTD Pressure (dbar)
13Light intensityOptical transmittance (%)
14Inc.TimeIncubation time (hour)
15Spike 13CSpiked 13C solution (µM)
16Filt. VolFiltering volume (mL)
17POCPOC (µg/L)
18POC_FLAGFlag of POC (for explanation see Quality flags)
1913CRatio of 13C (atom%)
2013C_FLAGFlag of 13C (for explanation see Quality flags)
21DICTotal dissolved inorganic carbon (µmol/kg)
22SalinitySalinity (PSU)
23Chl.aChlorophyll a. quantity (µg/L)
24DensitySeawater density (kg/L)
25DICTotal dissolved inorganic carbon (µmol/L)
26POCPOC (mg/m3)
2713C0Particulate organic carbon (13C) before incubation (atom%)
2813CxsRatio of 13C (atom%) - 13C0 (atom%)
2913CswConcentration of 13C of ambient seawater with a tracer (%)
30d-POCDelta POC/hour (mgC/m3/h)
31PP AveAverage of delta POC/day (mgC/m3/day)
32Integrated PPInitial Primary Production (mgC/m2/day)
33PBd-POC/Chl.a (mgC/mg chl.a/h)
34RemarksStation number (refer to CTD cast table of cruise report)

about 29,30,31)
For calculation, please see Cruise Report.

about 32)
The equation to be used in the calculations.
ex.) 10m to 20m
Integrated PP = (PP Ave at 10m + PP Ave at 20m)×(20m-10m)/2 + more shallow than Integrated PP at 10m

about 33)
PB = d-POC/Chl.a

Primary Productivity Data Sheet Format (SIS : simulated in-situ incubation)

Format information describes column no., column heading mnemonic and comments of simulated in-situ incubation primary productivity data sheet in MR08-05.
Missing value is presented by -999.

Column No.Column Heading MnemonicComments
1CruiseIDCruiseID
2STNNBRStation name
3CASTNOCast number (refer to CTD cast table of cruise report)
4Inc.TypeIncubation method (SIS : simulated in-situ incubation)
5UTC DateCTD start UTC date (refer to CTD cast table of cruise report)
6UTC TimeCTD start UTC time (refer to CTD cast table of cruise report)
7LatitudeCTD start position Latitude north degree (refer to CTD cast table of cruise report)
8LongitudeCTD start position Longitude east degree (refer to CTD cast table of cruise report)
9BTLNBRBottle identification number
10BTLNBR_FLAGBottle quality flag (for explanation see Quality flags)
11CTD DepthCTD Depth (m)
12CTD PRSCTD Pressure (dbar)
13Light intensityOptical transmittance (%)
14Inc.TimeIncubation time (hour)
15Spike 13CSpiked 13C solution (µM)
16Filt. VolFiltering volume (mL)
17POCPOC (µg/L)
18POC_FLAGFlag of POC (for explanation see Quality flags)
1913CRatio of 13C (atom%)
2013C_FLAGFlag of 13C (for explanation see Quality flags)
21DICTotal dissolved inorganic carbon (µmol/kg)
22SalinitySalinity (PSU)
23Chl.aChlorophyll a. quantity (µg/L)
24DensitySeawater density (kg/L)
25DICTotal dissolved inorganic carbon (µmol/L)
26POCPOC (mg/m3)
2713C0Particulate organic carbon(13C) before incubation (atom%)
2813CxsRatio of 13C - 13C0 (atom%)
2913CswConcentration of 13C of ambient seawater with a tracer(%)
30d-POCDelta POC/hour (mgC/m3/h)
31PP AveAverage of delta POC/day (mgC/m3/day)
32Integrated PPInitial Primary Production (mgC/m2/day)
33PBd-POC/Chl.a (mgC/mg chl.a/h)
34RemarksStation number (refer to CTD cast table of cruise report)

about 29,30,31)
For calculation, please see Cruise Report.

about 32)
The equation to be used in the calculations.
ex.) 10m to 20m
Integrated PP = (PP Ave at 10m + PP Ave at 20m)×(20m-10m)/2+more shallow than Integrated PP at 10m

about 33)
PB = d-POC/Chl.a

: MR08-05
: Processed (DMO)-QCed
: JAMSTEC
Flags of data quality control are as follows.

Related Information

Update History

2013-08-29
An observation data was registerd.
2012-10-26
An observation data was registerd.