MIRAI MR09-03 Leg2 Primary Production

Last Modified: 2018-02-24
: MR09-03 Leg2
: Processed (DMO)-QCed
: JAMSTEC
: POC
:
BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PRIMARY PRODUCTION
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PHOTOSYNTHESIS

For Using Data

Shigeto Nishino (JAMSTEC)
See Terms and Conditions about constrain of use.
See Terms and Conditions about data citation.
Instrument:
CN mass spectrometer
Primary Production Data during MR09-03 Leg2 cruise were obtained by the following methods : - Simulated in-situ incubation method (SIS) See Data List for available data at each station. Water sampling, incubation, and devices and tracers for analysis for each method are outlined below. For further information, please see Cruise Report.
Simulated in-situ incubation (SIS)
1) Vertical sampling : Niskin
2) Surface sampling : Bucket
3) Sampling layer : 5
4) Tracer : NaH13CO3, K15NO3, 15NH4Cl
5) Incubation period : 3hours
6) Filtration : Whatman GF/F 25mm filter was used at dark place.
7) Preservation : Filters were kept to freeze at -20degC and dried
 in the oven at 45degC.
8) Preservation period of frozen filter paper : within 45 days
9) Analysis place : MIRAI
10) Analysis device : CN mass spectrometer
11) Analysis method : Dumas method, Mass spectrometry
 CN mass spectrometer system equipped with R/V Mirai can measure stable isotope
 ratios of 13C and 15N comprised in liquid, solid, and gas states of
 biological or biogenic samples, simultaneously and continuously.
 This system consists of two devices, preprocessing equipment “ROBOPLEP-SL” and
 stable isotope ratio mass spectrometer “EUROPA20-20”.
 
 (1) ROBOPLEP-SL
 A tin capsule containing the sample falls into the combustion tube and is
 converted in the presence of oxygen to CO2, N2, NOx and H2O.
 An elemental copper stage reduces NOx, a MgClO4 trap removes water vapour,
 a switchable Carbosorb trap can be used to remove CO2 (for 15N only analyses)
 and a GC column separates CO2 from N2 (allowing dual isotope analysis).
 And then, it is introduced into the “EUROPA20-20”.
 
 (2) EUROPA20-20
 CO2 and N2 are collided with thermion and ionized in the high vacuum ion source.
 When the generated ions are accelerated by constant voltage and pass through
 the analysis tube, differences in mass (m) and electric charge (z) of isotope
 ions make the different orbits by the magnetic field in the analysis tube.
 Thus, isotopes can be separated by the displacement of the orbits.
 These signals are converted into the frequency at the detector, and transmitted
 to control PC. Blank and drift corrections are conducted on the control software.
 
 see flow diagram. MR09-03_pp_ANCA-SL PDF file
 
(1) ROBOPLEP-SL
Manufacturer : SerCon Ltd. (former PDZ Europa Ltd.)
Instruments : ANCA-SL ROBOPREP-SL
S/N : 17001-051
Sample Range Solids/Liquids : 10 to 1000 µgN, 10 to 1000 µgC.
Autosampler : 60 position pneumatic autosampler that takes (standard) capsules with up to 47mm in diameter.

(2) EUROPA 20-20
Manufacturer : SerCon Ltd. (former PDZ Europa Ltd.)
Instruments : ANCA-SL EUROPA 20-20
S/N : 9007-075
Analyzer and Analysis tube : 120° extended geometry with an 11 cm radius magnetic sector
Resolution : m/Δm=95 (N2) 10% valley definition
Sensitivity : Inside Vacuum level is 4×10-6mbar in an atmosphere of helium
 20 nmol CO2
 15 nmol N2
Abundance Sensitivity : Inside Vacuum level is 4×10-6mbar in an atmosphere of helium
 30 ppm for CO2 at 4×10-6mbar in continuous flow mode.
 5 ppm for N2 at 4×10-6mbar in continuous flow mode.

(3) Precision
All specifications are for n=5 samples.
It is a natural amount and five time standard deviation of the analysis as for amount 100 µg of the sample.
13C (0.2 ‰)
15N (0.5 ‰)

(4) Data processing
Device control and processing soft : ANCA ver.3.5 (former PDZ Europa Ltd.)
Fully compatible with Windows 3.1 or Windows 95.

(5) Reference material
The third-order reference materials whose data values were decided by the second reference materials (IAEA-N-1, IAEA-N-2, and IAEA-CH-6) dealt in International Atomic Energy Agency (IAEA) were used.
In this cruise, there is an observation log sheet at the time of the data acquisitions.
If necessary, please contact us from "Contact Us" above.
: MR09-03 Leg2
: Processed (DMO)-QCed
: JAMSTEC
: POC
:
BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PRIMARY PRODUCTION
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PHOTOSYNTHESIS

Observation Map

  1. Clicking the icon displays a balloon with observation information.
Imagery reproduced from ...

Data List

File names
MR09-03_leg2_pp_SIS.csv
MR09-03_leg2_pp_SIS_nh4.csv
MR09-03_leg2_pp_SIS_no3.csv
Observation List
The list of observation is shown as follows.
Observation Time and Date Lat. [°] Lon. [°]
04 2009-09-11 00:00 73.0000 -167.9800
10 2009-09-13 00:00 74.6100 -170.9100
15 2009-09-15 00:00 76.6400 -165.6800
25 2009-09-17 00:00 76.0100 -156.4000
33 2009-09-19 00:00 77.5200 -150.0000
35 2009-09-20 00:00 78.9900 -151.6500
58 2009-09-26 00:00 74.5100 -154.1100
62 2009-09-28 00:00 71.7400 -155.1400
75 2009-09-30 00:00 72.8800 -157.6500
83 2009-10-05 00:00 74.4400 -165.7300
: MR09-03 Leg2
: Processed (DMO)-QCed
: JAMSTEC

Primary Productivity Data Sheet Format

Format information describes column no., column heading mnemonic and comments of simulated in-situ incubation
primary productivity data sheet in MR09-03 Leg2.
Missing value is presented by -999.
Column No.Column Heading MnemonicComments
1CruiseIDCruiseID
2STNNBRStation name
3CASTNOCast number (refer to CTD cast table of cruise report)
4Inc.TypeIncubation method (SIS : simulated in-situ incubation)
5UTC DateCTD start UTC date (refer to CTD cast table of cruise report)
6UTC TimeCTD start UTC time (refer to CTD cast table of cruise report)
7LatitudeCTD start position Latitude north degree (refer to CTD cast table of cruise report)
8LongitudeCTD start position Longitude east degree (refer to CTD cast table of cruise report)
9BTLNBRBottle identification number
10CTD DepthCTD Depth (m)
11Light intensityOptical transmittance (%)
12Inc.TimeIncubation time (hour)
13Bottle nameSample name
14Spike 13CSpiked 13C solution (µM)
15Filt. VolFiltering volume (mL)
16POCPOC (µg)
17POC_FLAGFlag of POC (for explanation see Quality flags)
1813CRatio of 13C (atom%)
2913C_FLAGFlag of 13C (for explanation see Quality flags)
20DICTotal dissolved inorganic carbon (µmol/kg)
21SalinitySalinity (PSU)
22Chl.aChlorophyll a. quantity (µg/L)
23DensitySeawater density (kg/L)
24DICTotal dissolved inorganic carbon (µmol/L)
25POCPOC (mg/m3)
2613C013C ratio of zero time blank (atom%)
2713CxsRatio of 13C - 13C0 (atom%)
2813CswConcentration of 13C of ambient seawater with a tracer(%)
29d-POCDelta POC/hour (mgC/m3/h)
30PBd-POC/Chl.a (mgC/mg chl.a/h)
31RemarksComment of incubation, filtration and analysis.

about 14)
Amount of 13C in which 10% of Total dissolved inorganic carbon in seawater was added as tracer.

about 26)
Bottle name 0time data (Natural abundance) or 1.084atom%

about 28)
13Csw=(DIC×0.011+Spike 13C)/(DIC+Spike 13C)×100
0.011:Ratio of 13C included in the Total dissolved inorganic carbon


about 29)
d-POC=1.025×13Cxs×POC/(13Csw-13C0)/Inc.Time
1.025 : 13C Stable Isotope discrimination factor

about 30)
PB = d-POC/Chl.a

Regenerated Production Data Sheet Format

Format information describes column no., column heading mnemonic and comments of simulated in-situ incubation
regenerated production data sheet in MR09-03.
Missing value is presented by -999.

Column No.Column Heading MnemonicComments
1CruiseIDCruiseID
2STNNBRStation number
3CASTNOCast number (refer to CTD cast table of cruise report)
4Inc.TypeIncubation method (SIS : simulated in-situ incubation)
5UTC DateCTD start UTC date (refer to CTD cast table of cruise report)
6UTC TimeCTD start UTC time (refer to CTD cast table of cruise report)
7LatitudeCTD start position Latitude degree (refer to CTD cast table of cruise report)
8LongitudeCTD start position Longitude degree (refer to CTD cast table of cruise report)
9BTLNBRBottle identification number
10CTD DepthCTD Depth (m)
11Light intensityOptical transmittance (%)
12Inc.TimeIncubation time (hour)
13Bottle nameSample name
14Spike 15NH4Spiked 15N solution (µM)
15Filt. VolFiltering volume (mL)
16PONPON (µg)
17PON_FLAGFlag of PON (for explanation see Quality flags)
1815NRatio of 15N (atom%)
1915N_FLAGFlag of 15N (for explanation see Quality flags)
20TINDissolved Nitrate, Nitrite, Ammonium(µmol/kg)
21SalinitySalinity (PSU)
22Chl.aChlorophyll a. quantity (µg/L)
23DensitySeawater density (kg/L)
24TINDissolved Nitrate, Nitrite, Ammonium (µmol/L)
25PONPON (mg/m3)
2615N015N of natural abundance (atom%)
2715NxsRatio of 15N - 15N0 (atom%)
2815NenrConcentration of 15N of ambient seawater with a tracer (%)
29d-NNH4 uptake rate (mgN/m3/h)
30NBNH4 uptake rate per Chlorophyll a.(mgN/mg chl.a/h)
31RemarksComment of incubation, filtration and analysis.

about 14)
Amount of 15N in which 10% of Total Nitrogen in seawater was added as tracer.

about 26)
Natural abundance 0.366 atom%

about 28)
15Nenr=(Spike 15NH4+TIN×0.366/100)/(Spike 15NH4+TIN)×100-15N0

about 29)
d-N=PON×15Nxs/15Nenr/Inc.Time

about 30)
NB=d-N/Chl.a

New Production Data Sheet Format

Format information describes column no., column heading mnemonic and comments of simulated in-situ incubation
new production data sheet in MR09-03.
Missing value is presented by -999.
Column No.Column Heading MnemonicComments
1CruiseIDCruiseID
2STNNBRStation number
3CASTNOCast number (refer to CTD cast table of cruise report)
4Inc.TypeIncubation method (SIS : simulated in-situ incubation)
5UTC DateCTD start UTC date (refer to CTD cast table of cruise report)
6UTC TimeCTD start UTC time (refer to CTD cast table of cruise report)
7LatitudeCTD start position Latitude degree (refer to CTD cast table of cruise report)
8LongitudeCTD start position Longitude degree (refer to CTD cast table of cruise report)
9BTLNBRBottle identification number
10CTD DepthCTD Depth (m)
11Light intensityOptical transmittance (%)
12Inc.TimeIncubation time (hour)
13Bottle nameSample name
14Spike 15NO3Spiked 15N solution (µM)
15Filt. VolFiltering volume (mL)
16PONPON (µg)
17PON_FLAGFlag of PON (for explanation see Quality flags)
1815NRatio of 15N (atom%)
1915N_FLAGFlag of 15N (for explanation see Quality flags)
20TINDissolved Nitrate, Nitrite, Ammonium(µmol/kg)
21SalinitySalinity (PSU)
22Chl.aChlorophyll a. quantity (µg/L)
23DensitySeawater density (kg/L)
24TINDissolved Nitrate, Nitrite, Ammonium (µmol/L)
25PONPON (mg/m3)
2615N015N of natural abundance (atom%)
2715NxsRatio of 15N - 15N0 (atom%)
2815NenrConcentration of 15N of ambient seawater with a tracer (%)
29d-NNO3 uptake rate (mgN/m3/h)
30NBNO3 uptake rate per Chlorophyll a.(mgN/mg chl.a/h)
31RemarksComment of incubation, filtration and analysis.

about 14)
Amount of 15N in which 10% of Total Nitrogen in seawater was added as tracer.

about 26)
Natural abundance 0.366 atom%

about 28)
15Nenr=(Spike 15NO3+TIN×0.366/100)/(Spike 15NO3+TIN)×100-15N0

about 29)
d-N=PON×15Nxs/15Nenr/Inc.Time

about 30)
NB=d-N/Chl.a
: MR09-03 Leg2
: Processed (DMO)-QCed
: JAMSTEC
Flags of data quality control are as follows.

Related Information

Update History

2018-02-24
An observation data was registerd.
2013-08-29
An observation data was registerd.
2012-09-28
An observation data was registerd.