MIRAI MR10-05 Leg2 Primary Production

Last Modified: 2018-02-24
: MR10-05 Leg2
: Processed (PI)
: JAMSTEC
: POC
:
BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PRIMARY PRODUCTION
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PHOTOSYNTHESIS

For Using Data

Shigeto Nishino (JAMSTEC)
See Terms and Conditions about constrain of use.
See Terms and Conditions about data citation.
Instrument:
CN mass spectrometer
 
・25 Nov. 2013 add "New production" and "Regenerated production" data were added.  
Primary, new and regenerated production data during MR10-05 Leg2 cruise were obtained by the following methods :
 - Simulated in-situ incubation method (SIS)
 Water sampling, incubation, and devices and tracers for analysis for each method
 are outlined below.
 For further information, please see Cruise Report.
1) Vertical sampling : Niskin
2) Surface sampling : Bucket
3) Sampling layer : 7-8
4) Tracer : NaH13CO3, K15NO3, 15NH4Cl
5) Incubation period : 3hours (except Stn.126 and Stn.165 for primary production)
6) Filtration : Whatman GF/F 25mm filter was used at dark place.
7) Preservation : Filters were kept to freeze at -20degC and dried
 in the oven at 45degC.
8) Preservation period of frozen filter paper : -
9) Analysis place : MIRAI
10) Analysis device : CN mass spectrometer
11) Analysis method : Mass spectrometry refer to Lee and Whitledge (2005)
 CN mass spectrometer system equipped with R/V Mirai can measure stable isotope
 ratios of 13C and 15N comprised in liquid, solid, and gas states of
 biological or biogenic samples, simultaneously and continuously.
 This system consists of two devices, preprocessing equipment “ANCA-SL, former ROBOPLEP-SL” and
 stable isotope ratio mass spectrometer “20-20, former EUROPA20-20”.
(1) ANCA-SL A tin capsule containing the sample falls into the combustion tube and is converted in the presence of oxygen to CO2, N2, NOx and H2O. An elemental copper stage reduces NOx, a MgClO4 trap removes water vapour, a switchable Carbosorb trap can be used to remove CO2 (for 15N only analyses) and a GC column separates CO2 from N2 (allowing dual isotope analysis). And then, it is introduced into the “20-20”.
(2) 20-20 CO2 and N2 are collided with thermion and ionized in the high vacuum ion source. When the generated ions are accelerated by constant voltage and pass through the analysis tube, differences in mass (m) and electric charge (z) of isotope ions make the different orbits by the magnetic field in the analysis tube. Thus, isotopes can be separated by the displacement of the orbits. These signals are converted into the frequency at the detector, and transmitted to control PC. Blank and drift corrections are conducted on the control software. see flow diagram. PDF file
(1) ANCA-SL
Manufacturer : SerCon Ltd. (former PDZ Europa Ltd.)
Instruments : ANCA-SL
S/N : 17001-051
Sample Range Solids/Liquids : 10 to 1000 µgN, 10 to 1000 µgC.
Autosampler : 60 position pneumatic autosampler that takes (standard) capsules with up to 47mm in diameter.

(2) 20-20
Manufacturer : SerCon Ltd. (former PDZ Europa Ltd.)
Instruments : 20-20
S/N : 9007-075
Analyzer and Analysis tube : 120° extended geometry with an 11 cm radius magnetic sector
Resolution : m/Δm=95 (N2) 10% valley definition
Sensitivity : Inside Vacuum level is 4×10-6mbar in an atmosphere of helium
 20 nmol CO2
 15 nmol N2
Abundance Sensitivity : Inside Vacuum level is 4×10-6mbar in an atmosphere of helium
 30 ppm for CO2 at 4×10-6mbar in continuous flow mode.
 5 ppm for N2 at 4×10-6mbar in continuous flow mode.
(3) Precision
All specifications are for n=5 samples.
It is a natural amount and five time standard deviation of the analysis as for amount 100 µg of the sample.
13C (0.2 ‰)
15N (0.5 ‰)

Lee and Whitledge (2005) Primary and new production in the deep Canada Basin during summer 2002. Polar Biol 28 : 190-197
: MR10-05 Leg2
: Processed (PI)
: JAMSTEC
: POC
:
BIOSPHERE > AQUATIC ECOSYSTEMS > PLANKTON > PHYTOPLANKTON
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PRIMARY PRODUCTION
BIOSPHERE > ECOLOGICAL DYNAMICS > ECOSYSTEM FUNCTIONS > PHOTOSYNTHESIS

Observation Map

Imagery reproduced from ...

Data List

File names
MR10-05_leg2_np_SIS_no3.csv
MR10-05_leg2_pp_SIS.csv
MR10-05_leg2_rp_SIS_nh4.csv
: MR10-05 Leg2
: Processed (PI)
: JAMSTEC

Primary Productivity Data Sheet Format (MR10-05_leg2_pp_SIS)

Format information describes column no., column heading mnemonic and comments of simulated in-situ incubation
primary productivity data sheet in MR10-05 Leg2.
Missing value is presented by -999.

Column No.Column Heading MnemonicComments
1 CruiseID CruiseID
2 STNNBR Station name
3 CASTNO Cast number (refer to CTD cast table of cruise report)
4 Inc.Type Incubation method (SIS : simulated in-situ incubation)
5 UTC Date CTD start UTC date (refer to CTD cast table of cruise report)
6 UTC Time CTD start UTC time (refer to CTD cast table of cruise report)
7 Latitude CTD start position Latitude north degree (refer to CTD cast table of cruise report)
8 Longitude CTD start position Longitude east degree (refer to CTD cast table of cruise report)
9 BTLNBR Bottle identification number
10 BTLNBR_FLAG Bottle identification number(for explanation see Definition of Quality Control Flags for Bottle Data)
11 CTD Depth CTD Depth (m)
12 CTD PRS CTD Pressure (dbar)
13 Light intensity Incubation light intensity relative to the surface (%)
14 Inc.Time Incubation time (hour)
15 Bottle name Sample name
16 Spike 13C Spiked 13C solution (µM)
17 Filt. Vol Filtering volume (mL)
18 POC POC (µg)
19 POC_FLAG Flag of POC (for explanation see Quality flags)
20 13C Ratio of 13C (atom%)
21 13C_FLAG Flag of 13C (for explanation see Quality flags)
22 DIC Total dissolved inorganic carbon (µmol/kg)
23 Salinity Salinity (PSU)
24 Chl.a Chlorophyll a. quantity (µg/L)
25 Density Seawater density (kg/L)
26 DIC Total dissolved inorganic carbon (µmol/L)
27 POC POC (mgC/m3)
28 13C0 Natural abundance of 13C (atom%)
29 13Cxs Ratio of 13C - 13C0 (atom%)
30 13Csw Concentration of 13C of ambient seawater with a tracer (%)
31 d-POC Delta POC/hour (mgC/m3/h)
32 PB d-POC/Chl.a (mgC/mg chl.a/h)
33 Remarks Comment of incubation, filtration and analysis.

about 16)
Amount of 13C in which 10% of Total dissolved inorganic carbon in seawater was added as tracer.
about 28)
Bottle name 0time average data (Natural abundance).
about 30)
13Csw=(DIC×0.011+Spike 13C)/(DIC+Spike 13C)×100
0.011:Ratio of 13C included in the Total dissolved inorganic carbon
about 31)
d-POC=1.025×13Cxs×POC/(13Csw-13C0)/Inc.Time
1.025 : 13C Stable Isotope discrimination factor
about 32)
PB = d-POC/Chl.a

Regenerated Productivity Data Sheet Format (MR10-05 Leg2_rp_SIS_nh4)

Format information describes column no., column heading mnemonic and comments of simulated in-situ incubation regenerated productivity data sheet in MR10-05 Leg2.
Missing value is presented by -999.

Column No.Column Heading MnemonicComments
1 CruiseID CruiseID
2 STNNBR Station number
3 CASTNO Cast number (refer to CTD cast table of cruise report)
4 Inc.Type Incubation method (SIS : simulated in-situ incubation)
5 UTC Date CTD start UTC date (refer to CTD cast table of cruise report)
6 UTC Time CTD start UTC time (refer to CTD cast table of cruise report)
7 Latitude CTD start position Latitude degree (refer to CTD cast table of cruise report)
8 Longitude CTD start position Longitude degree (refer to CTD cast table of cruise report)
9 BTLNBR Bottle identification number
10 BTLNBR_FLAG Bottle quality flag (for explanation seeDefinition of Quality Control Flags for Bottle Data)
11 CTD Depth CTD Depth (m)
12 CTD PRS CTD Pressure (dbar)
13 Light intensity Optical transmittance (%)
14 Inc.Time Incubation time (hour)
15 Bottle name Sample name
16 Spike 15NH4 Spiked 15N solution (µM)
17 Filt. Vol Filtering volume (mL)
18 PON PON (µg)
19 PON_FLAG Flag of PON (for explanation see Quality flags)
20 15N Ratio of 15N (atom%)
21 15N_FLAG Flag of 15N (for explanation see Quality flags)
22 Salinity Salinity (PSU)
23 Chl.a Chlorophyll a. quantity (µg/L)
24 Density Seawater density (kg/L)
25 PON PON (mg/m3)
26 15N0 15N of natural abundance (atom%)
27 15Nxs Ratio of 15N - 15N0 (atom%)
28 15Nenr Concentration of 15N of ambient seawater with a tracer (%)
29 d-N NH4 uptake rate (mgN/m3/h)
30 NB NH4 uptake rate per Chlorophyll a.(mgN/mg chl.a/h)
31 Remarks Comment of incubation, filtration and analysis.

about 16)
Amount of 15N in which 10% of Total Nitrogen in seawater was added as tracer.
about 26)
Bottle name 0time average data (Natural abundance).
about 28)
15Nenr=(Spike 15NH4+NH4×0.366/100)/(Spike 15NH4+NH4)×100-15N0
Natural abundance 0.366 atom%
about 29)
d-N=PON×15Nxs/15Nenr/Inc.Time
about 30)
NB=d-N/Chl.a

New Productivity Data Sheet Format (MR10-05_leg2_np_SIS_no3)

Format information describes column no., column heading mnemonic and comments of simulated in-situ incubation
new production data sheet in MR10-05 Leg2.
Missing value is presented by -999.

Column No.Column Heading MnemonicComments
1 CruiseID CruiseID
2 STNNBR Station number
3 CASTNO Cast number (refer to CTD cast table of cruise report)
4 Inc.Type Incubation method (SIS : simulated in-situ incubation)
5 UTC Date CTD start UTC date (refer to CTD cast table of cruise report)
6 UTC Time CTD start UTC time (refer to CTD cast table of cruise report)
7 Latitude CTD start position Latitude degree (refer to CTD cast table of cruise report)
8 Longitude CTD start position Longitude degree (refer to CTD cast table of cruise report)
9 BTLNBR Bottle identification number
10 BTLNBR_FLAG Bottle quality flag (for explanation seeDefinition of Quality Control Flags for Bottle Data)
11 CTD Depth CTD Depth (m)
12 CTD PRS CTD Pressure (dbar)
13 Light intensity Optical transmittance (%)
14 Inc.Time Incubation time (hour)
15 Bottle name Sample name
16 Spike 15NO3 Spiked 15N solution (µM)
17 Filt. Vol Filtering volume (mL)
18 PON PON (µg)
19 PON_FLAG Flag of PON (for explanation see Quality flags)
20 15N Ratio of 15N (atom%)
21 15N_FLAG Flag of 15N (for explanation see Quality flags)
22 Salinity Salinity (PSU)
23 Chl.a Chlorophyll a. quantity (µg/L)
24 Density Seawater density (kg/L)
25 PON PON (mg/m3)
26 15N0 15N of natural abundance (atom%)
27 15Nxs Ratio of 15N - 15N0 (atom%)
28 15Nenr Concentration of 15N of ambient seawater with a tracer (%)
29 d-N NO3 uptake rate (mgN/m3/h)
30 NB NO3 uptake rate per Chlorophyll a.(mgN/mg chl.a/h)
31 Remarks Comment of incubation, filtration and analysis.

about 16)
Amount of 15N in which 10% of Total Nitrogen in seawater was added as tracer.
about 26)
Bottle name 0time average data (Natural abundance).
about 28)
15Nenr=(Spike 15NO3+NO3×0.366/100)/(Spike 15NO3+NO3)×100-15N0
Natural abundance 0.366 atom%
about 29)
d-N=PON×15Nxs/15Nenr/Inc.Time
about 30)
NB=d-N/Chl.a
: MR10-05 Leg2
: Processed (PI)
: JAMSTEC
Flags of data quality control are as follows.

Related Information

Update History

2018-02-24
An observation data was registerd.
2013-12-03
An observation data was registerd.
2013-07-24
An observation data was registerd.