Concentrations of phytoplankton pigment were sampled from subsurface layer (<100 m). Filter samples were stored in a deep-freezer (-80˚C) until analysis on land. Pigments were extracted in DMF with 10 min bath sonication and soaked overnight in a freezer (-20˚C). The pigment extract was analyzed following to the HPLC method of van-Heaukelem and Thomas (2001) using a C8 Zorbax Eclipse column maintained at 60˚C.
Upon consultation in advance with the chief of investigation and the person(s) in charge of research issues who gathered that data, we request that the text of the results material contain a statement to the effect that it was obtained during the R/V Mirai cruise of MR17-05C, the Chief Scientist, Shigeto Nishino (JAMSTEC), and the following Principal Investigators (PIs) for gathering the data.
Chief Scientist Shigeto Nishino Japan Agency for Marine - Earth Science and Technology (JAMSTEC) 2-15 Natsushima, Yokosuka, Kanagawa 237-0061, Japan Tel: +81-46-867-9487, Fax: +81-46-867-9437 E-mail: email@example.com
PI Amane Fujiwara (JAMSTEC)
Please also mention that this cruise was supported by the Arctic Challenge for Sustainability (ArCS) Project, which was funded by the Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT), and the Global Change Observation Mission-Climate (GCOM-C) mission of Japan Aerospace Exploration Agency (JAXA).
HPLC 1300 series (Agilent Technology)
Microsoft Excel fromat. Details are descrebed in data file "MR1705C_HPLC_pigmentconcentration.xlsx"